Fluorescent bioinspired albumin/polydopamine nanoparticles and their interactions with Escherichia coli cells

• Eloïse Equy,
• Jordana Hirtzel,
• Sophie Hellé,
• Béatrice Heurtault,
• Eric Mathieu,
• Morgane Rabineau,
• Vincent Ball and
• Lydie Ploux

Beilstein J. Nanotechnol. 2023, 14, 1208–1224, doi:10.3762/bjnano.14.100

• Standard and high-resolution fluorescence confocal laser scanning microscopy (CLSM) were used to determine the localization of the fluorescent BSA/PDA NPs with a BSA/DA ratio of 10 in bacterial cells. One colony of E. coli was transferred from the agar plate into fresh liquid LB and incubated at 37 °C

Suspension feeding in Copepoda (Crustacea) – a numerical model of setae acting in concert

• Alexander E. Filippov,
• Wencke Krings and
• Stanislav N. Gorb

Beilstein J. Nanotechnol. 2023, 14, 603–615, doi:10.3762/bjnano.14.50

• field of filtration technologies. Keywords: adhesion; confocal laser scanning microscopy (CLSM); feeding efficiency; feeding structures; mechanical properties; Introduction Particle capture mechanisms are common in a huge variety of aquatic animals, such as polychaetes, bryozoans, bivalves, sponges

• chose the copepod Centropages hamatus (Lilljeborg, 1853). This species belongs to the Calanoida, where filter feeding is the derived condition [19][20][29][42][43][44][45][46][47][48][49][50][51][52][53][54]. For this species (Figure 1), previous confocal laser scanning microscopy (CLSM) studies on the

Dry under water: air retaining properties of large-scale elastomer foils covered with mushroom-shaped surface microstructures

• Matthias Mail,
• Stefan Walheim,
• Thomas Schimmel,
• Wilhelm Barthlott,
• Stanislav N. Gorb and
• Lars Heepe

Beilstein J. Nanotechnol. 2022, 13, 1370–1379, doi:10.3762/bjnano.13.113

• samples by confocal laser scanning microscopy (CLSM) showed the expected persistence of the air layer (see Figure 2). The images taken directly after the submersion of the sample show an almost perfectly flat air–water interface. After two weeks under water the same sample still kept an air layer and was

• , the silvery shine indicates the kept air. c) SEM image of the surface structure of the Salvinia leaf. d) SEM image of the MSM. Confirmation of the persistence of the air layer in low water depth and analysis of the shape of the air–water interface by confocal laser scanning microscopy (CLSM

Coordination-assembled myricetin nanoarchitectonics for sustainably scavenging free radicals

• Xiaoyan Ma,
• Haoning Gong,
• Kenji Ogino,
• Xuehai Yan and
• Ruirui Xing

Beilstein J. Nanotechnol. 2022, 13, 284–291, doi:10.3762/bjnano.13.23

• to effectively protect cells from damage. DCFH-DA was used to probe ROS in cells, which showed no fluorescence signal without ROS, while it turned to highly fluorescent 2′7′-dichlorofluorescein after interacting with ROS in cells. As shown in the confocal laser scanning microscopy (CLSM) images, the

Bacterial safety study of the production process of hemoglobin-based oxygen carriers

• Axel Steffen,
• Yu Xiong,
• Radostina Georgieva,
• Ulrich Kalus and
• Hans Bäumler

Beilstein J. Nanotechnol. 2022, 13, 114–126, doi:10.3762/bjnano.13.8

• CCD method produces nearly uniform, peanut-shaped particles. The size distribution determined by dynamic light scattering (DLS) was 759 ± 25 nm. Confocal laser scanning microscopy (CLSM) images confirmed this size range (Figure 1C). Scanning electron microscopy (SEM) images of particles produced with

Polarity in cuticular ridge development and insect attachment on leaf surfaces of Schismatoglottis calyptrata (Araceae)

• Venkata A. Surapaneni,
• Tobias Aust,
• Thomas Speck and
• Marc Thielen

Beilstein J. Nanotechnol. 2021, 12, 1326–1338, doi:10.3762/bjnano.12.98

• changes Figure 1 shows S. calyptrata leaves at their different ontogenetic stages (Figure 1a) and the corresponding confocal laser scanning microscopy (CLSM) observations (Figure 1b–f) on leaf microstructures. A schematic representation of the leaves and the corresponding locations of smooth and ridged

Fusion of purple membranes triggered by immobilization on carbon nanomembranes

• René Riedel,
• Natalie Frese,
• Fang Yang,
• Martin Wortmann,
• Raphael Dalpke,
• Daniel Rhinow,
• Norbert Hampp and
• Armin Gölzhäuser

Beilstein J. Nanotechnol. 2021, 12, 93–101, doi:10.3762/bjnano.12.8

• histidine-tag at the extracellular side of a PM mutant (c-His PM). The functionalization and the resulting hybrid membrane were examined by atomic force microscopy (AFM), scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), confocal laser scanning microscopy (CLSM), and infrared

PEG/PEI-functionalized single-walled carbon nanotubes as delivery carriers for doxorubicin: synthesis, characterization, and in vitro evaluation

• Shuoye Yang,
• Zhenwei Wang,
• Yahong Ping,
• Yuying Miao,
• Yongmei Xiao,
• Lingbo Qu,
• Lu Zhang,
• Yuansen Hu and
• Jinshui Wang

Beilstein J. Nanotechnol. 2020, 11, 1728–1741, doi:10.3762/bjnano.11.155

• detect and observe the fluorescence signal in cells. Further, confocal laser scanning microscopy (CLSM) was utilized to observe the internalization. Cells were seeded into 6-well plates and incubated for 24 h. After co-incubation in 2 mL of medium containing free DOX or DOX-loaded nanocarrier

Silver-decorated gel-shell nanobeads: physicochemical characterization and evaluation of antibacterial properties

• Marta Bartel,
• Katarzyna Markowska,
• Marcin Strawski,
• Krystyna Wolska and
• Maciej Mazur

Beilstein J. Nanotechnol. 2020, 11, 620–630, doi:10.3762/bjnano.11.49

• ) analysis were performed with a Zeiss Merlin field-emission SEM instrument. Transmission electron microscopy images were collected with a Zeiss Libra 120 EFTEM. Confocal laser scanning microscopy (CLSM) imaging of bacterial biofilms was performed with a Nikon AIR MP microscope equipped with a 60×, NA 1.4

Multilayer capsules made of weak polyelectrolytes: a review on the preparation, functionalization and applications in drug delivery

• Varsha Sharma and
• Anandhakumar Sundaramurthy

Beilstein J. Nanotechnol. 2020, 11, 508–532, doi:10.3762/bjnano.11.41

• force [13] using confocal laser scanning microscopy (CLSM), atomic force microscopy (AFM), and reflection interference contrast microscopy (RICM). Fabrication conditions such as the type of polymer (e.g., thicker layers are formed by PEs having lower charge density) [14], concentration of the polymer

Brome mosaic virus-like particles as siRNA nanocarriers for biomedical purposes

• Alfredo Nuñez-Rivera,
• Pierrick G. J. Fournier,
• Danna L. Arellano,
• Ana G. Rodriguez-Hernandez,
• Rafael Vazquez-Duhalt and
• Ruben D. Cadena-Nava

Beilstein J. Nanotechnol. 2020, 11, 372–382, doi:10.3762/bjnano.11.28

• the formula (L·w2)/2 where L and w stand for tumor length and width, respectively. Cellular uptake of CCMV and BMV viruses. (a) Confocal laser scanning microscopy (CLSM) images of MCF-7 cells treated with virus–NanoOrange (green channel). (b) Representative flow cytometry data (c) and the statistical

Microfluidics as tool to prepare size-tunable PLGA nanoparticles with high curcumin encapsulation for efficient mucus penetration

• Nashrawan Lababidi,
• Valentin Sigal,
• Aljoscha Koenneke,
• Konrad Schwarzkopf,
• Andreas Manz and
• Marc Schneider

Beilstein J. Nanotechnol. 2019, 10, 2280–2293, doi:10.3762/bjnano.10.220

• . Besides a specific surface chemistry with a tendency to avoid the interaction with mucins, NPs smaller than the pore size of mucus [54] need to be applied to avoid the size-filtering mechanism [5][10]. A confocal laser scanning microscopy (CLSM)-based set up was used to study the penetration of NPs

High-tolerance crystalline hydrogels formed from self-assembling cyclic dipeptide

• Yongcai You,
• Ruirui Xing,
• Qianli Zou,
• Feng Shi and
• Xuehai Yan

Beilstein J. Nanotechnol. 2019, 10, 1894–1901, doi:10.3762/bjnano.10.184

• cross-linked networks are also the foundation for a range of biomedical and nanotechnological applications. Interior structure and crystal pattern The fibrillar structure and three-dimensional fibrous network of the C-WY hydrogel were further investigated by confocal laser scanning microscopy (CLSM

Toxicity and safety study of silver and gold nanoparticles functionalized with cysteine and glutathione

• Barbara Pem,
• Igor M. Pongrac,
• Lea Ulm,
• Ivan Pavičić,
• Valerije Vrček,
• Darija Domazet Jurašin,
• Marija Ljubojević,
• Adela Krivohlavek and
• Ivana Vinković Vrček

Beilstein J. Nanotechnol. 2019, 10, 1802–1817, doi:10.3762/bjnano.10.175

• using confocal laser scanning microscopy (CLSM) in the reflection contrast mode. In cells treated with CYS-AgNPs, GSH-AgNPs and CYS-AuNPs, accumulated NPs were clearly visible intracellularly, while it was not possible to detect such accumulation in the case of treatment with GSH-AuNPs (see Figure 5 and

• marked with asterisk (*) differ significantly from the negative control (P < 0.05). The NP uptake within L929 cells (a–d) compared to untreated control cells (e–h) as visualized by reflection mode confocal laser scanning microscopy (CLSM). The images show maximum intensity Z-projections of cells stained

A new bioinspired method for pressure and flow sensing based on the underwater air-retaining surface of the backswimmer Notonecta

• Matthias Mail,
• Adrian Klein,
• Horst Bleckmann,
• Anke Schmitz,
• Torsten Scherer,
• Peter T. Rühr,
• Goran Lovric,
• Robin Fröhlingsdorf,
• Stanislav N. Gorb and
• Wilhelm Barthlott

Beilstein J. Nanotechnol. 2018, 9, 3039–3047, doi:10.3762/bjnano.9.282

• the setae indeed occurs if pressure changes, confocal laser scanning microscopy (CLSM) was used (see Experimental section). In the projection through a stack of CLSM images, the setae as well as the reflecting air–water interface in between the setae could be monitored (Figure 6c). Cross sections

• ) and in each segment the number of setae was counted in three randomly selected areas of 0.5 mm2. CLSM investigations of the pressure behavior The air–water interface was analyzed by confocal laser scanning microscopy (CLSM, Leica TSM 500). The laser beam of the microscope was reflected at the air

The effect of flexible joint-like elements on the adhesive performance of nature-inspired bent mushroom-like fibers

• Elliot Geikowsky,
• Serdar Gorumlu and
• Burak Aksak

Beilstein J. Nanotechnol. 2018, 9, 2893–2905, doi:10.3762/bjnano.9.268

• of M-3180 (BJB Enterprises, E = 8.89 MPa). The joint-like elements are made of TC-9445 for the stiff joint, M-3180 for the soft joint, and Vytaflex-30 (Smooth-On, E = 0.45 MPa) for the very soft joints. All the materials were cured at ambient for three days before testing. a) Confocal laser scanning

• microscopy (CLSM) of a lateral view of discoidal (mushroom-shaped) adhesive hairs in a male ladybird beetle. Differences in the autofluorescence indicate the presence and distribution of different materials. Blue regions (transitions from the hair shaft to the tip structure) indicate portions of the soft

Development of an advanced diagnostic concept for intestinal inflammation: molecular visualisation of nitric oxide in macrophages by functional poly(lactic-co-glycolic acid) microspheres

• Kathleen Lange,
• Christian Lautenschläger,
• Maria Wallert,
• Stefan Lorkowski,
• Andreas Stallmach and
• Alexander Schiller

Beilstein J. Nanotechnol. 2017, 8, 1637–1641, doi:10.3762/bjnano.8.163

• -loaded microspheres. Confocal laser scanning microscopy (CLSM) images of activated (A) and inactivated (B) NO550-loaded polymeric microspheres in DPBS including fluorescence emission spectra (C) of the microspheres are shown. UV-irradiated SNP (10 mM, 2 min, 254 nm) was used for 2 minutes to activate

Uptake of the proteins HTRA1 and HTRA2 by cells mediated by calcium phosphate nanoparticles

• Olga Rotan,
• Katharina N. Severin,
• Simon Pöpsel,
• Alexander Peetsch,
• Melisa Merdanovic,
• Michael Ehrmann and
• Matthias Epple

Beilstein J. Nanotechnol. 2017, 8, 381–393, doi:10.3762/bjnano.8.40

• electron microscopy. Various cell types (HeLa, MG-63, THP-1, and hMSC) were incubated with fluorescently labelled proteins alone or with protein-loaded cationic and anionic nanoparticles. The cellular uptake was followed by light and fluorescence microscopy, confocal laser scanning microscopy (CLSM), and

• nm, emission: 460 nm) channels. Confocal laser scanning microscopy (CLSM) was performed on a Leica SP5 confocal inverse CLSM. The cells were stained with DAPI (nucleus) and Cell mask™ (cell membrane) to indicate the cellular uptake of the nanoparticles. To elucidate the uptake mechanism, several

On the pathway of cellular uptake: new insight into the interaction between the cell membrane and very small nanoparticles

• Claudia Messerschmidt,
• Daniel Hofmann,
• Anja Kroeger,
• Katharina Landfester,
• Volker Mailänder and
• Ingo Lieberwirth

Beilstein J. Nanotechnol. 2016, 7, 1296–1311, doi:10.3762/bjnano.7.121

• to have a detailed look at the very moment of particle uptake, revealing a remarkable dependency of the observed morphologies on particle size. Alongside the EM analysis we applied confocal laser scanning microscopy (CLSM), biochemical assays for LDH and ATP, flow cytometry, Caspase-3 western

Functional diversity of resilin in Arthropoda

• Jan Michels,
• Esther Appel and
• Stanislav N. Gorb

Beilstein J. Nanotechnol. 2016, 7, 1241–1259, doi:10.3762/bjnano.7.115

• with a larger number of arthropod species, it will represent the first reliable method that specifically identifies resilin. The development and improvement of methods applying techniques such as micromechanical testing, atomic force microscopy and confocal laser scanning microscopy (CLSM) have

Silica micro/nanospheres for theranostics: from bimodal MRI and fluorescent imaging probes to cancer therapy

• Shanka Walia and
• Amitabha Acharya

Beilstein J. Nanotechnol. 2015, 6, 546–558, doi:10.3762/bjnano.6.57

• liver cancer cells, NIH-3T3 mouse fibroblast cells, and 4T1 mouse breast cancer cells. Confocal laser scanning microscopy (CLSM) images indicated a successful bioconjugation of silica-coated QDs and MQDs with a bio-anchored membrane. Again, Salgueiriño-Maceira et al. [44] reported a new class of

Hematopoietic and mesenchymal stem cells: polymeric nanoparticle uptake and lineage differentiation

• Ivonne Brüstle,
• Thomas Simmet,
• Gerd Ulrich Nienhaus,
• Katharina Landfester and
• Volker Mailänder

Beilstein J. Nanotechnol. 2015, 6, 383–395, doi:10.3762/bjnano.6.38

• (magnetite) embedded (PLLA–Fe). Confocal laser scanning microscopy (cLSM) was performed to determine the intracellular localization of the nanoparticles. The images in Figure 2 clearly show that all particle types were indeed internalized by the cells and not only attached to the cell surface. hHSCs showed a

• , apoptotic, dead) and taking the percentage of gated cells in the respective gate. Signals from APC-coupled antibodies were analyzed by overlays of several signals in die APC channel. Confocal laser scanning microscopy (cLSM) Confocal laser scanning microscopy was performed to validate the intracellular

Imaging the intracellular degradation of biodegradable polymer nanoparticles

• Anne-Kathrin Barthel,
• Martin Dass,
• Melanie Dröge,
• Jens-Michael Cramer,
• Daniela Baumann,
• Markus Urban,
• Katharina Landfester,
• Volker Mailänder and
• Ingo Lieberwirth

Beilstein J. Nanotechnol. 2014, 5, 1905–1917, doi:10.3762/bjnano.5.201

• period of 14 days, primarily by means of transmission electron microscopy (TEM), in order to demonstrate their degradation. Furthermore, confocal laser scanning microscopy (CLSM) and flow cytometry were used to monitor the nanoparticle load of individual cells. As a probe we chose tailor-made PLLA

• cultures. All values are triplicates with the error bars representing the standard deviation. Confocal laser scanning microscopy (CLSM) Confocal laser scanning microscopy (CLSM) was applied to demonstrate the intracellular distribution of nanoparticles over the period of 14 days. As described in [26], for

• confocal laser scanning microscopy (CLSM), MSCs were seeded in α-MEM solution at a density of 20 000 cells in ibiTreat µ-slides (IBIDI, Germany). On the following day particles were added to the medium at a concentration of 300 μg·mL−1. After an incubation time of 24 h, the supernatant was removed and

Dry friction of microstructured polymer surfaces inspired by snake skin

• Martina J. Baum,
• Lars Heepe,
• Elena Fadeeva and
• Stanislav N. Gorb

Beilstein J. Nanotechnol. 2014, 5, 1091–1103, doi:10.3762/bjnano.5.122

• elevated, so the snake can generate propulsion due to the interlocking of its microstructure with surface asperities. The results of the study of the snake skin’s microstructure by using atomic force microscopy (AFM) and confocal laser scanning microscopy (CLSM) showed that the anisotropic geometry of the

Fibrillar adhesion with no clusterisation: Functional significance of material gradient along adhesive setae of insects

• Stanislav N. Gorb and
• Alexander E. Filippov

Beilstein J. Nanotechnol. 2014, 5, 837–845, doi:10.3762/bjnano.5.95

• revealed by confocal laser scanning microscopy (CLSM). This gradient is hypothesized to be an evolutionary optimization enhancing adaptation of adhesive pads to rough surfaces, while simultaneously preventing setal clusterisation. Such an optimisation presumably increases the performance of the adhesive